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Cells were lysed in 100 L ice cold 8 M Urea/50 mM Tris HCl lysis buffer (Fisher Scientific) with 1:100 of Halt Cocktail Inhibitor, EDTA-free (100X) (Thermo Scientific)

Importantly, there was an apparent improvement in cardiac function in the cAtg3-KO mice following treatment [8]

Physically, you might feel weak, dizzy, or notice a strange tingling or numbness in your hands and feet